Background: Clostridium difficile is a major cause of antibiotic-associated diarrhea. There are the reports about increasing incidence rate of C. difficile in adult in Europe, North America and Korea. However the epidemiology of C. difficile infections (CDI) in children is currently unknown in Korea. The purpose of this study was to evaluate the incidence of pediatric CDI in Korea. Methods: We analysed the medical records of patients under the age of 18, diagnosed with CDI at Severance Children`s Hospital from January 2009 to July 2017. CDI was diagnosed when a hospitalized child with diarrhea or colitis had a positive toxigenic stool culture result. The incidence rate was compared between early period (2009-2012) and late period (2013-2017). Results: We identified 101 patients with CDI. During the study period, the incidence rate of CDI in children under the age of 18 years was 8.79 cases per 10,000 admitted patients. The incidence of CDI was 2.52 cases per 10,000 admitted patients in early period group, and increased to 14.04 cases per 10,000 admitted patients in late period group (p0.005). The groups of 16-18 and 10-15 years of age had the first (14.47/10,000) and second (10.67/10,000) highest rates of CDI admissions, the third highest rate was in the group that was 1-4 year of age (9.44/10,000). The group that was 5-9 years of age had the lowest rates of CDI admissions (5.06/10,000). Conclusion: An increase of CDI in children has been observed during recent 8 years. Awareness of CDI in children is important for conducting appropriate CDI control in hospitals, fueling the development of new therapeutic options. Further nationwide studies are needed for detailed surveillance and effective CDI management.